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Eco Real-Time PCR

 With the Eco system, Real-Time PCR technology is now within reach of individual researchers. Large, expensive thermal cyclers that take up an entire workspace are replaced with an affordable, compact system and Netbook computer that fit easily in any lab. Delivering unsurpassed data quality for 40-cycle runs in as little as 40 minutes, the Eco system revolutionizes qPCR accessibility for both new and experienced Real-Time PCR users.

eco_full_set_introduction.jpg   

The flexible Eco system is an open platform that supports all chemistries and Real-Time PCR applications, including absolute quantification by standard curve, relative quantification using the ΔΔCq method with support for multiple reference gene normalization, allelic discrimination by end-point fluorescence, and gene scanning and genotyping by High Resolution Melt (HRM) curve analysis.

High-performance Real-Time PCR system. Includes Eco Real-Time instrument, Netbook computer pre-installed with Eco software including the HRM module, Eco sample loading dock, USB flash drive with Eco software, and a starter pack consisting of 10 plates, 40 seals, and one evaluation kit.

 

Specifications

Instrument

 

Thermal system

Single Peltier-based system

Block format

48-well block

Consumables

48-well Eco plates and optical Eco adhesive seals

Accessories

Eco sample loading dock

Sample volumes

5–20µl validated (optimized for standard 20µl protocols)

Average block ramp rate

5.5°C/sec

Temperature range

35–100°C

Temperature uniformity

± 0.1°C

Optical system

LED excitation (452–486 nm and 542–582 nm), four emission filters (505–545 nm, 562–596 nm, 604–644 nm, and 665–705 nm) and CCD camera

Calibrated dyes at shipment

SYBR, FAM, HEX, ROX, Cy5: factory-calibrated. Additional dyes within wavelength range of Eco filters are supported with no additional calibration required for implementation.

Data collection

Data collected in all four filters for all wells regardless of plate setup; plate setup for data analysis can be altered after run completion.

Melt curve analysis supports continuous data acquisition in a single channel, providing increased data point collection and reduced run times

Real-Time PCR run time (40 cycles)

Less than 40 minutes 

Electrical

Voltage: 120VAC=10%
Nominal current draw: 8A
Frequency: 50/60 Hz=1%
Peak Power 500VA, typical power is 180VA

Software

Eco System Software supports all chemistries and a variety of applications, including absolute quantification, relative quantification, allelic discrimination, and high resolution melt curve analysis (HRM)

Warranty

12-month warranty (includes parts and labor)

 

Instrument Physical

 

Instrument dimensions

12.6" closed (height)
14.5" open (height)
13.6" (depth)
12.2" (width)

32 cm closed (height)
36.8 cm open (height)
34.5 cm (depth)
31 cm (width)

Weight

30 lbs (13.6 Kg)

 

Performance

 

Sensitivity

1 copy

Dynamic range

9 logs linear range

Precision

Discriminates 5,000 and 10,000 template copies with 99% confidence

 

 

 

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