Loading... Please wait...
Popular Brands
Our Newsletter
Fast Cycling
So fast and powerful..they could be the first SuperMixes to face mandatory drug testing.
PerfeCTa® FastMixes® set a new standard for high efficiency qPCR results with a significantly faster protocol for increased productivity.
About PerfeCTa® FastMixes®
PerfeCTa® qPCR FastMixes® are 2X concentrated, ready-to-use reaction cocktails that deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using either fast or conventional cycling protocols. Rapid cycling is achieved by instant activation of AccuFast™ Taq DNA polymerase coupled with rapid polymerization kinetics. The result is qPCR in as little as 33 minutes. PerfeCTa® qPCR FastMixes® are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye.
Protocol Comparison: Conventional vs. Fast
|
|
Conventional Reagents |
PerfeCTa® FastMix® |
|
Activation 95°C |
600-900s |
20s |
|
PCR Cycling 95°C/60°C |
15s/60s |
1-3s/20s |
|
Time for 40 cycles (excl. ramp time) |
52 min |
14 min |
|
Total Run Time: iCycler iQ® |
80-103 min (4-6 runs/8 hr shift) |
40 min (12 runs/8 hr shift) |
|
Total Run Time: |
72-82min (5-6 runs/8 hr shift) |
33 min (14 runs/8 hr shift) |
Fast Cycling qPCR
Get up to 3x faster cycle times and 5x greater sensitivity.
Probe Detection
|
|
|
|
|
|
|
|
Detection using SYBR Green I
Fast Cycling One-Step qRT-PCR
Quanta Biosciences is introducing the first One-Step Fast qRT-PCR kits.
One-Step qRT-PCR Kits
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|