Description

AccuStart Taq DNA Polymerase is a recombinant Taq DNA polymerase preparation which contains monoclonal antibodies that bind to the polymerase and keep it inactive before PCR thermal cycling. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active Taq DNA polymerase. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. Activated AccuStart Taq DNA polymerase possesses 5'→3' DNA polymerase activity and a double-strand specific 5'→3' exonuclease. The polymerase does not have 3'-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Contents

• AccuStart Taq DNA polymerase - 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers.
• 10X PCR Buffer - 0.2M Tris-HCl (pH 8.4), 0.5 M KCl
• 50 mM magnesium chloride - 50 mM MgCl2

Storage conditions

AccuStart Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.