Fast EvaGreen^®qPCR Master Mix is a PCR reagent suitable for both qPCR using a fast cycling protocol and high resolution melt curve (HRM) analysis. The reagent system uses two of our breakthrough PCR technologies, EvaGreen^® qPCR dye and Cheetah^TM Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

• EvaGreen^® Dye: EvaGreen^® DNA-binding dye is the next generation dye from Biotium for both real-time PCR detection and HRM. The dye selectively binds to dsDNA via a novel "release-on-demand" mechanism that ensures low PCR inhibition and permits HRM application at below saturation dye concentration. Because the dye is spectrally similar to FAM or SYBR^® Green I, the dye is compatible with all commercial qPCR instruments. Moreover, unlike SYBR^® Green I and SYBR GreenER^?, EvaGreen^® dye is extremely stable. Finally, EvaGreen^® dye is nonmutagenic and noncytotoxic.

• Cheetah^TM Taq: Cheetah^TM Taq is a new, chemically modified hotstart DNA polymerase. Unlike Amplitaq Gold^®, Cheetah^TM Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum^® Taq, Cheetah^TM Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah^TM Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold^®, ensuring that the master mix maintain its peak performance following storage at low temperature.

Our Fast EvaGreen^® Master Mix has been tested on a variety of templates, including templates that are short (<100 bp), long (>700bp), AT-rich, GC-rich or of genomic origin. The results show, Fast EvaGreen^® Master Mix is highly robust and superior to SYBR-based master mixes from other suppliers. Because we invented both the dye and enzyme technologies, we can offer you the master mix at a price that is among the most competitive. The top performance and competitive price of our master mix present a value unrivaled by similar products from other suppliers.

Fast EvaGreen^® qPCR Master Mix is also available in 500-reaction size (cat#31003-1) and 5,000-reaction size (cat#31003-2).