Fast Probe Master Mix is designed for quantitative real time analysis of DNA samples from various sources using probe-based detection. Although optimized for AllGlo™ probe system, this master mix is also suitable for other probe systems including TaqManÒ and Molecular BeaconÒ.

The AllGlo™ probe is a simple fluorogenic probe without quencher or MGB. AllGlo™ probes generate two light emitting labels per probe, giving more intense fluorescence than traditional TaqManÒ probes. AllGlo™ probes are available in six colours: MAR, JUP, SAT, URA, NEP and PLU which match with the absorption and emission wavelength of FAM, HEX, TAMRA, ROX, Cy5 and Quasar 670, respectively. These six AllGlo™ dye labels are compatible with most real-time instruments and generally require no special calibration. For detailed probe design guidelines refer to AllGlo™ user’s manual (www.allelogic.com).

This probe based master mix offers strong signal and high sensitivity, which make gene quantitation and viral load assessment possible even for the most challenging samples such as tissue, blood or serum. The wide range of reporter dye choices enables multi-gene detection in a single tube and provides reliable results for multiplex applications such as SNP gene typing and pathogen detection.

Another critical component of the master mix is Cheetah™ Taq, our proprietary chemically-modified hot-start DNA Polymerase. Cheetah^TM Taq is a new, chemically modified hotstart DNA polymerase. Unlike AmpliTaq Gold^®, Cheetah^TM Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum^® Taq, Cheetah^TM Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah^TM Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold^®, ensuring that the master mix maintain its peak performance following storage at low temperature

Fast Probe Master Mix is formulated for qPCR using a fast cycling protocol, albeit, it is also compatible with qPCR using a regular cycling protocol.

Product Protocol

References:

1. Xin X and Mao F. AllGlo™ Probe: Highly Specific Homo-labeled Probe Technology for Real-Time qPCR. 3^rd International qPCR Symposium. 26^th – 30^th March 2007, in Freising-Weihenstephan, ISBN-13: 978-3-00-020385-5.

2. Xin X. A closed-tube real-time PCR system for simultaneous pathogen detection and mutation scanning. 2008. 11^th WPCCID PS3-078