Description

PerfeCTa® SYBR® Green FastMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR systems that do not require an internal reference dye. This unique combination of proprietary buffer, stabilizers, and AccuFast™ Taq DNA polymerase deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR® Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. AccuFast Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a proprietary mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (> 48 hours at room temperature). Similar to our AccuStart Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200 bp is complete in less than 20s at 60°C.

Real-Time PCR Instrument Compatibility

• Roche LightCycler™ 480
• Qiagen/Corbett Rotor-Gene® Q
• Qiagen/Corbett Rotor-Gene® 6000
• Qiagen/Corbett Rotor-Gene® 3000
• Eppendorf Mastercycler® ep realplex
• Eppendorf Mastercycler® ep realplex2 s
• Cepheid SmartCycler®
• Bio-Rad CFX96™
• Bio-Rad CFX384™
• Bio-Rad/MJ Opticon®
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Chromo4™
• Bio-Rad MiniOpticon™

Contents

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuFast Taq DNA Polymerase, SYBR Green I dye, and stabilizers.

Storage conditions

PerfeCTa® SYBR Green FastMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.