Features & Benefits

• High yield, high sensitivity
• Highly specific amplification
• Improved reproducibility and assay precision

Description

PerfeCTa® qPCR SuperMix, Low ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Real-Time PCR Instrument Compatibility

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Stratagene Mx3000P®
• Stratagene Mx3005P™
• Stratagene Mx4000™

Contents

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

Storage conditions

PerfeCTa® qPCR SuperMix, Low ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.