Features & Benefits

• High yield, high sensitivity
• Highly specific amplification
• Superior reproducibility and precision

Description

PerfeCTa® qPCR SuperMix, ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR on Applied Biosystems 7000, 7300, 7700, or 7900 instruments. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Real-Time PCR Instrument Compatibility

• Applied Biosystems 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900HT
• Applied Biosystems 7900 HT Fast
• Applied Biosystems StepOne™
• Applied Biosystems StepOnePlus™

Contents

• 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, ROX Reference Dye, and stabilizers.

Storage conditions

PerfeCTa® qPCR SuperMix, ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.